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PostPosted: 04 Aug 2009, 07:52 
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They're commonly used for shooting glasses too.


True. I have a pair.

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PostPosted: 04 Aug 2009, 11:39 
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Bono wears a pair of yellow sunglasses all of the time. Yeah.


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PostPosted: 04 Aug 2009, 11:57 
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[quote](by reducing perceived light they make your iris open more, yet all damaging effects of sun are unfiltered ...)

....one of the reasons I've never used sunglasses.

I stick with nature's method: squinting. :onethumb
That's why they need to be UV A/B blocking.


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PostPosted: 04 Aug 2009, 14:29 
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[quote][quote](by reducing perceived light they make your iris open more, yet all damaging effects of sun are unfiltered ...)

....one of the reasons I've never used sunglasses.

I stick with nature's method: squinting. :onethumb
That's why they need to be UV A/B blocking.
exactly but according to my local glasses retailer, yellow sunglasses aren't up to the standard enough aka even if they advertise some degree of UV A/B blocking it won't ever be enough...

edit: and he sells all sorts of glasses , among them said yellow ones


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PostPosted: 04 Aug 2009, 16:40 
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To be honest, I thought yellow glasses were supposed to reduce eyestrain of short-term sudden variations in brightness (such as when shooting, or being on a ski slope) rather than be a 'cure all' or standardised sunglasses.

I don't really favour yellow glasses anyway - although I did quite like the light brown lenses my last sunglasses had... after I got used to them. They weren't perfect, but I tested them once by sticking them in the beamline of a UV/Vis spectrophotometer - they maxed out the absorbance below 350nm, so they were certainly blocking a lot of UV. :)


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PostPosted: 04 Aug 2009, 21:45 
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...according to my local glasses retailer, yellow sunglasses aren't up to the standard enough aka even if they advertise some degree of UV A/B blocking it won't ever be enough...
Note that the only yellow glasses in that ad are the bonus "Night Vision" pair. The yellow ones aren't intended as "sunglasses". The actual sunglasses in that ad are more of a amber/brown color.


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PostPosted: 04 Aug 2009, 22:19 
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I tested them once by sticking them in the beamline of a UV/Vis spectrophotometer

So have you worked in just about every field that begets movie villians? ;) :P

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PostPosted: 05 Aug 2009, 09:56 
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So have you worked in just about every field that begets movie villians? ;) :P

Um... no.


A fairly typical flavin UV/Vis spectrum - it's not quite 'typical' as that would have maxima at 350 and 450nm. It's a thiol oxidase, which I've never worked on personally. The big peak that maxes at 280nm is the protein, the 'double bump' with maxima at ~370nm and 450nm is the characteristic flavin optical spectrum. Oxidised flavin looks yellow. Anything below about 370nm isn't visible as it's UV - putting my glasses in the beamline makes the readings below 350nm hit 10 (which is the maximum absorbance that the spec I used registers...) The amount of protein-bound flavin is actually fairly low in this example... I doubt that more than 10% of the protein has a cofactor bound.


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PostPosted: 05 Aug 2009, 21:09 
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A fairly typical flavin UV/Vis spectrum - it's not quite 'typical' as that would have maxima at 350 and 450nm. It's a thiol oxidase, which I've never worked on personally. The big peak that maxes at 280nm is the protein, the 'double bump' with maxima at ~370nm and 450nm is the characteristic flavin optical spectrum. Oxidised flavin looks yellow. Anything below about 370nm isn't visible as it's UV - putting my glasses in the beamline makes the readings below 350nm hit 10 (which is the maximum absorbance that the spec I used registers...) The amount of protein-bound flavin is actually fairly low in this example... I doubt that more than 10% of the protein has a cofactor bound.

I confess, you lost me at "flavin".

Just kiddin around about the movie stuff. It must be pretty handy to be able to find out for yourself if a product's claims are total BS or not. Wish I had that ability for a few things I've purchased.....

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PostPosted: 05 Aug 2009, 21:55 
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I confess, you lost me at "flavin".

Just kiddin around about the movie stuff. It must be pretty handy to be able to find out for yourself if a product's claims are total BS or not. Wish I had that ability for a few things I've purchased.....

Sorry.

Biochemist geek mode: off.

No worries about the movie stuff, I know you're kiddin'.

Yeah, it's pretty good to be able to test stuff at times.

There are lots of things I want to test that I can't, however... :(


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